The Phytophthora capsici Genome Project

Phytophthora capsici is a non-indigenous US pathogen. It was first reported in the US in 1922 on chili peppers in New Mexico and spread to vegetable production areas in Colorado and Florida in the 1930's and 1940's, affecting tomatoes, eggplants, squash, and melons.

NCGR (Stephen Kingsmore, PI), along with biologists at University of Tennessee and Ohio State University, is funded by USDA/NSF to sequence the P. capsici genome. The rationale for these studies is:

1. P. capsici is a devastating pathogen of vegetable crops of national economic importance
2. P. capsici is an excellent genetic model. This project will create broadly applicable resources for gene models and population genetic studies of oomycete biology and hemibiotroph-induced disease
3. 454 sequencing technology will be evaluated and benchmarked for de novo and re-sequencing in the largest genome studied to date (65MB).

In collaboration and support from DOE's JGI sequencing group, the aims are to use novel 454 Life Sciences sequencing technology to generate:

1. 20X draft genome sequence of the vegetable pathogen Phytophthora capsici
2. 2X coverage resequencing in 4 outbred isolates
3. A catalog of single nucleotide variation.

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P. capsici Genome Project